Electrochemical detection of redox molecules secreted by Pseudomonas aeruginosa – Part 1: Electrochemical signatures of different strains

During infections, fast identification of the microorganisms is critical to improve patient treatment and better manage antibiotics use. Electrochemistry exhibits several advantages for rapid diagnostic: it enables easy, cheap in situ analysis redox molecules most liquids. In this work, culture supernatants different Pseudomonas aeruginosa strains (including PAO1 its isogenic mutants PAO1ΔpqsA, PA14, PAK CHA) were analyzed by square wave voltammetry on glassy carbon electrode during bacterial growth. The obtained voltamograms shown complex traces exhibiting numerous peaks with potential repartitions current amplitudes depending studied bacterium and/or growth time. Among them, some clearly associated well-known toxin Pyocyanin (PYO) autoinducer Quinolone Signal (PQS). Other observed that are not yet attributed known secreted species. Each electrochemical response (number peaks, peak amplitude) can be interpreted as a fingerprint or “ID-card” strain may implemented bacteria identification.